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Cytokeratin 14 Polyklonaler Antikörper

Cytokeratin 14 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10143

Synonyme

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Cytokeratin 14 Polyc (CL488-10143)

    IF Staining of HepG2 using CL488-10143

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Cytokeratin 14 antibody (CL488-10143) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated Cytokeratin 14 Polyc (CL488-10143)

    FC experiment of A431 using CL488-10143

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 14 (CL488-10143) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCA431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10143 bindet in IF, FC (Intra) Cytokeratin 14 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cytokeratin 14 fusion protein Ag0188
Vollständiger Name keratin 14
Berechnetes Molekulargewicht 472 aa, 52 kDa
Beobachtetes Molekulargewicht 47-50 kDa
GenBank-ZugangsnummerBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Cytokeratin 14 antibody CL488-10143Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen