Cystatin B Monoklonaler Antikörper

Cystatin B Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G9B8

Kat-Nr. : 66812-1-Ig

Synonyme

CPI B, CST6, CSTB, Cystatin B, cystatin B (stefin B), EPM1, PME, Stefin B, STFB

---

Galerie der Validierungsdaten

• 

  WB analysis of U-937 using 66812-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 66812-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66812-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 66812-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, humanes Speichelgewebe, THP-1-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66812-1-Ig bindet in WB, IF, ELISA Cystatin B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cystatin B fusion protein Ag21291
• Vollständiger Name: cystatin B (stefin B)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11-14 kDa
• GenBank-Zugangsnummer: BC003370
• Gene symbol: CSTB
• Gene ID (NCBI): 1476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.