Cyclin D3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin D3 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2C6

Rezensionen (1)

Kat-Nr. : 66357-1-Ig

Synonyme

CCND3, Cyclin D3, G1/S specific cyclin D3

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Galerie der Validierungsdaten

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  WB analysis of Raji using 66357-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66357-1-Ig

  WB result of Cyclin D3 antibody (66357-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

• 

  WB analysis of HEK-293 using 66357-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66357-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66357-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 66357-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HEK-293-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66357-1-Ig bindet in WB, IHC,ELISA Cyclin D3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin D3 fusion protein Ag22825
• Vollständiger Name: cyclin D3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC011616
• Gene symbol: CCND3
• Gene ID (NCBI): 896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janardhan (Verified Customer) (08-22-2020)

Antibody works well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Medulloblastoma