Cyclin A2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin A2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

CloneNo.

1C6C11

Rezensionen (1)

Kat-Nr. : 66391-1-Ig

Synonyme

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Galerie der Validierungsdaten

• 

  WB analysis using 66391-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66391-1-Ig

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

• 

  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IF: See 2 publications below

Produktinformation

66391-1-Ig bindet in WB, IHC, IF, ELISA Cyclin A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin A2 fusion protein Ag12765
• Vollständiger Name: cyclin A2
• Berechnetes Molekulargewicht: 432 aa, 49 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC104783
• Gene symbol: CCNA2
• Gene ID (NCBI): 890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Publikationen

Species	Application	Title
human	IF	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta
human	WB	Oncogene; KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.; Authors - Y Zhong
human	IF	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
human	WB	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
human	WB	Cell Cycle; Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D.; Authors - Jinghua Yu
human	WB	Molecules; A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition; Authors - Yan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juri (Verified Customer) (01-20-2024)

Signal present after ICC, but expressing from all the cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 100
• Cell Tissue Type: cancer cells