Collagen Type XXVIII Polyklonaler Antikörper

Collagen Type XXVIII Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24401-1-AP

Synonyme

COL28, COL28A1, Collagen alpha 1(XXVIII) chain, Collagen Type XXVIII, collagen, type XXVIII, alpha 1

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Galerie der Validierungsdaten

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  WB analysis using 24401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 24401-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 24401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24401-1-AP bindet in WB, IF, ELISA Collagen Type XXVIII und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type XXVIII fusion protein Ag21459
• Vollständiger Name: collagen, type XXVIII, alpha 1
• Berechnetes Molekulargewicht: 1125 aa, 117 kDa
• Beobachtetes Molekulargewicht: 72 kDa, 100-117 kDa
• GenBank-Zugangsnummer: BC136892
• Gene symbol: COL28A1
• Gene ID (NCBI): 340267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).