COL4A2-specific Polyklonaler Antikörper

COL4A2-specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

66

Rezensionen (4)

Kat-Nr. : 55131-1-AP

Synonyme

COL4A2, Collagen alpha 2(IV) chain, Collagen Type IV, collagen, type IV, alpha 2

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Galerie der Validierungsdaten

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  WB analysis using 55131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55131-1-AP (COL4A2-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 55131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 55131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammahyperplasie-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 20 publications below
• IF: See 13 publications below

Produktinformation

55131-1-AP bindet in WB, IHC, IF, ELISA COL4A2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type IV, alpha 2
• Berechnetes Molekulargewicht: 168 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_001846
• Gene symbol: COL4A2
• Gene ID (NCBI): 1284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human		EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
	IF	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
human	IF	Biomaterials; Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy.; Authors - Menghao Shi
human	IHC	Biofabrication; Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin.; Authors - Lise De Moor
	IHC	Oxid Med Cell Longev; Pretreatment with Roxadustat (FG-4592) Attenuates Folic Acid-Induced Kidney Injury through Antiferroptosis via Akt/GSK-3β/Nrf2 Pathway.; Authors - Xue Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pankhuri (Verified Customer) (08-22-2022)

Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.

• Applications: Immunofluorescence
• Cell Tissue Type: NSC organoid

COL4A2-specific Antibody Immunofluorescence validation ( dilution) in NSC organoid (Cat no:55131-1-AP)

FH

Lindsey (Verified Customer) (05-24-2021)

BULLETPROOF ANTIBODY!

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human kidney (FFPE)

COL4A2-specific Antibody Immunohistochemistry,Immunofluorescence validation (1:500 dilution) in Human kidney (FFPE) (Cat no:55131-1-AP)

FH

Malak (Verified Customer) (02-24-2021)

This antibody works very well

• Applications: Western Blot

FH

Joshua (Verified Customer) (04-28-2019)

Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HMEC1

COL4A2-specific Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)