Collagen Type I Monoklonaler Antikörper

Collagen Type I Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9A7

Kat-Nr. : 67288-1-Ig

Synonyme

Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, collagen, type I, alpha 1, OI4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Kolongewebe, Hausschwein-Hautgewebe, Hausschwein-Lungengewebe, humanes Plazenta-Gewebe, humanes Zervixkarzinomgewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Kolonkarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67288-1-Ig bindet in WB, IHC, IF, ELISA Collagen Type I und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
In Publikationen genannte Reaktivitäthuman, Kaninchen
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name collagen, type I, alpha 1
Berechnetes Molekulargewicht139 kDa
Beobachtetes Molekulargewicht 120-130 kDa
GenBank-ZugangsnummerNM_000088
Gene symbol COL1A1
Gene ID (NCBI) 1277
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).

Protokolle

Produktspezifische Protokolle
WB protocol for Collagen Type I antibody 67288-1-IgProtokoll herunterladen
IHC protocol for Collagen Type I antibody 67288-1-IgProtokoll herunterladen
IF protocol for Collagen Type I antibody 67288-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
humanIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanIHC

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
WB,IHC

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
WB

Eur Respir J

Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.

Authors - Shengren Song
WB

Nat Commun

Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.

Authors - Yinan Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Niky (Verified Customer) (02-26-2024)

Arrived very quickly on ice. However, didnt work for my tissue type

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: endometrial lesion
FH

Udesh (Verified Customer) (12-22-2023)

Worked well for IF at 1:400

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: BM-MSCs
FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: human fibroblasts
FH

Ren Jie (Verified Customer) (06-22-2022)

Does not work for pepsin digested human collagen I

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Purified protein
FH

Hala (Verified Customer) (02-26-2021)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
FH

Hala (Verified Customer) (02-23-2021)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000