Collagen Type I Monoklonaler Antikörper
Collagen Type I Monoklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1E9A7
Kat-Nr. : 67288-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Hausschwein-Kolongewebe, Hausschwein-Hautgewebe, Hausschwein-Lungengewebe, humanes Plazenta-Gewebe, humanes Zervixkarzinomgewebe |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | humanes Kolonkarzinomgewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:2500-1:10000 |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 95 publications below |
IHC | See 26 publications below |
IF | See 25 publications below |
Produktinformation
67288-1-Ig bindet in WB, IHC, IF, ELISA Collagen Type I und zeigt Reaktivität mit Hausschwein, human
Getestete Reaktivität | Hausschwein, human |
In Publikationen genannte Reaktivität | human, Kaninchen |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | collagen, type I, alpha 1 |
Berechnetes Molekulargewicht | 139 kDa |
Beobachtetes Molekulargewicht | 120-130 kDa |
GenBank-Zugangsnummer | NM_000088 |
Gene symbol | COL1A1 |
Gene ID (NCBI) | 1277 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for Collagen Type I antibody 67288-1-Ig | Protokoll herunterladen |
IHC protocol for Collagen Type I antibody 67288-1-Ig | Protokoll herunterladen |
IF protocol for Collagen Type I antibody 67288-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma | ||
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Adv Sci (Weinh) Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Eur Respir J Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis. | ||
Nat Commun Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Niky (Verified Customer) (02-26-2024) | Arrived very quickly on ice. However, didnt work for my tissue type
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FH Udesh (Verified Customer) (12-22-2023) | Worked well for IF at 1:400
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FH Niklas (Verified Customer) (11-08-2022) | great staining with even a high dilution factor
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FH Ren Jie (Verified Customer) (06-22-2022) | Does not work for pepsin digested human collagen I
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FH Hala (Verified Customer) (02-26-2021) | very good
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FH Hala (Verified Customer) (02-23-2021) | very good
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