Chromogranin A Polyklonaler Antikörper

Chromogranin A Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10529

Synonyme

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie der Validierungsdaten

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  IF Staining of mouse pancreas using CL488-10529

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

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  IF Staining of mouse pancreas using CL488-10529

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

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  FC experiment of Neuro-2a using CL488-10529

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Chromogranin A (CL488-10529) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Pankreasgewebe
• Erfolgreiche Detektion in FC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10529 bindet in IF, FC (Intra) Chromogranin A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag0807
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: CHGA
• Gene ID (NCBI): 1113
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C