Chicken IgY Monoklonaler Antikörper

Chicken IgY Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Huhn

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E12G2

Kat-Nr. : 67405-1-Ig

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 67405-1-Ig

  100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hühner-IgY, Hühnerei

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67405-1-Ig bindet in WB, ELISA Chicken IgY und zeigt Reaktivität mit Huhn

• Getestete Reaktivität: Huhn
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Chicken IgY
• Beobachtetes Molekulargewicht: 180 kDa, 70 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).

Publikationen

Species	Application	Title
		Carbohydr Polym; Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y.; Authors - Hao Li