p21 Polyklonaler Antikörper

p21 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 28248-1-AP

Synonyme

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1

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Galerie der Validierungsdaten

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  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 28248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, DC2.4-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, Sp2/0-Zellen
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Produktinformation

28248-1-AP bindet in WB, IHC, IF, ELISA p21 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p21 fusion protein Ag28394
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (P21)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_001111099
• Gene symbol: Cdkn1a
• Gene ID (NCBI): 12575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

Publikationen

Species	Application	Title
mouse	WB	Bioact Mater; Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma; Authors - Yuhan Wang
mouse	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
mouse	IF	Dev Cell; Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.; Authors - Chang Xie
mouse	WB	Int J Mol Sci; Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis; Authors - Jiying Hou
		Int Immunopharmacol; Aflatoxin B1 exposure triggers inflammation and premature skin aging via ERMCS/Ca2+/ROS signaling cascade; Authors - Deyi Wu
mouse	WB	Ecotoxicol Environ Saf; Exploring the molecular mechanisms underlie the endoplasmic reticulum stress-mediated methylmercury-induced neuronal developmental damage; Authors - Jingjing Pan