Cd68 Polyklonaler Antikörper

Cd68 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (3)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

173

Rezensionen (2)

Kat-Nr. : 28058-1-AP

Synonyme

Cd68, CD68 antigen, gp110, Scard1

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 28058-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 28058-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 28058-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Mouse Cd68 (28058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausmilzgewebe
• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 35 publications below
• IHC: See 75 publications below
• IF: See 70 publications below
• FC: See 2 publications below

Produktinformation

28058-1-AP bindet in WB, IHC, IF, FC, ELISA Cd68 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cd68 fusion protein Ag27827
• Vollständiger Name: CD68 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_001291058
• Gene symbol: Cd68
• Gene ID (NCBI): 12514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publikationen

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	IF	J Pineal Res; Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.; Authors - Wei Liu
mouse	IF	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
	IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
mouse	IF	Acta Biomater; Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization; Authors - Yongzheng Li
mouse	IHC	Biomaterials; Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.; Authors - Ting Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

FH

Amelie (Verified Customer) (09-09-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse adrenal gland

Cd68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)