Caspase 3/p17/p19 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G4B2

Kat-Nr. : CL488-66470

Synonyme

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie der Validierungsdaten

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  IF Staining of human kidney using CL488-66470

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-66470, Clone: 2G4B2 ) at dilution of 1:100.

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  IF Staining of mouse brain using CL488-66470

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-66470, Clone: 2G4B2 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-66470

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-66470, Clone: 2G4B2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Nierengewebe, Maushirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66470 bindet in IF Caspase 3/p17/p19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 3/p17/p19 fusion protein Ag25029
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 19 kDa, 17 kDa
• GenBank-Zugangsnummer: BC016926
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.