Caspase 3 Rekombinanter Antikörper

Caspase 3 Rekombinant Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5G20

Kat-Nr. : 82202-1-RR

Synonyme

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 82202-1-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82202-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 82202-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Staurosporin behandelte Jurkat-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82202-1-RR bindet in WB, IHC, IF, FC, ELISA Caspase 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 17 kDa, 19 kDa
• GenBank-Zugangsnummer: NM_004346
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.