Caspase 1 Rekombinanter Antikörper

Caspase 1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

1O18

Kat-Nr. : 81482-1-RR

Synonyme

CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45

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Galerie der Validierungsdaten

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  WB analysis using 81482-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81482-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

81482-1-RR bindet in WB, IF, ELISA Caspase 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Caspase 1 fusion protein Ag11143
• Vollständiger Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Berechnetes Molekulargewicht: 404 aa, 45 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 35 kDa, 43-45 kDa
• GenBank-Zugangsnummer: BC062327
• Gene symbol: CASP1
• Gene ID (NCBI): 834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Scutellarin Rescued Mitochondrial Damage through Ameliorating Mitochondrial Glucose Oxidation via the Pdk-Pdc Axis; Authors - Ning Sheng
rat	WB,IF	Phytomedicine; Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells; Authors - Dong Wang
mouse	WB	Antioxidants (Basel); Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice; Authors - Jingfei Zhang
mouse	WB	Brain Res; Neuronal pyroptosis mediated by STAT3 in early brain injury after subarachnoid hemorrhage; Authors - Shengjie Tang
mouse	WB	Heliyon; LncRNA GAS5 downregulates NLRP3 inflammasome activation-mediated pyroptosis in sepsis-induced myocardial injury by targeting SIRT3/AMPKα; Authors - Minghao Luo
human,mouse	WB,IF	Biol Direct; RGS5 maintaining vascular homeostasis is altered by the tumor microenvironment; Authors - Peng Kong