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Calnexin Polyklonaler Antikörper

Calnexin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10427

Synonyme

calnexin, CANX, CNX, FLJ26570, IP90, P90

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Calnexin Polyclonal (CL488-10427)

    IF Staining of HepG2 using CL488-10427

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Calnexin antibody (CL488-10427) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Calnexin Polyclonal (CL488-10427)

    FC experiment of HeLa using CL488-10427

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Calnexin (CL488-10427) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10427 bindet in IF, FC (Intra) Calnexin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Calnexin fusion protein Ag0697
Vollständiger Name calnexin
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC003552
Gene symbol CANX
Gene ID (NCBI) 821
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Calnexin antibody CL488-10427Protokoll herunterladen
FC protocol for CL Plus 488 Calnexin antibody CL488-10427Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen