CYTSA Polyklonaler Antikörper

CYTSA Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 25390-1-AP

Synonyme

cytospin A, CYTSA, KIAA0376, SPECC1 like protein, SPECC1L

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 25390-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25390-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25390-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25390-1-AP

  IP Result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, Maushirngewebe, Neuro-2a-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

25390-1-AP bindet in WB, IP, IHC, IF, ELISA CYTSA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYTSA fusion protein Ag21896
• Vollständiger Name: cytospin A
• Berechnetes Molekulargewicht: 1117 aa, 125 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136471
• Gene symbol: CYTSA
• Gene ID (NCBI): 23384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

Publikationen

Species	Application	Title
mouse	WB,IF	Hum Mol Genet; In-frame deletion of SPECC1L microtubule association domain results in gain-of-function phenotypes affecting embryonic tissue movement and fusion events.; Authors - Jeremy P Goering
human	WB,IF	Hum Mol Genet; SPECC1L regulates palate development downstream of IRF6.; Authors - Everett G Hall
human	WB	Curr Biol; EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules; Authors - Takashi Hotta
human	WB,IF,IP	J Biol Chem; SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin; Authors - Virja Mehta