CYP2S1 Monoklonaler Antikörper

CYP2S1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3E5D4

Kat-Nr. : 68198-1-Ig

Synonyme

CYP2S1, CYPIIS1, Cytochrome P450 2S1

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Galerie der Validierungsdaten

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  WB analysis using 68198-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68198-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCCIT-Zellen, A549-Zellen, Hausschwein-Kolongewebe, Hausschwein-Rektumgewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68198-1-Ig bindet in WB, ELISA CYP2S1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP2S1 fusion protein Ag4605
• Vollständiger Name: cytochrome P450, family 2, subfamily S, polypeptide 1
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC033691
• Gene symbol: CYP2S1
• Gene ID (NCBI): 29785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cytochrome P450 (CYP) family of isoenzymes are involved in the metabolism of diverse endogenous compounds and detoxification of exogenous agents. Cytochrome P450 2S1 (CYP2S1) is one 'orphan' CYP, and is particularly expressed in epithe-lial cells of tissues which are exposed to the environment such as the skin, respiratory, gastrointes-tinal, and urinary tracts. CYP2S1 is associated primarily with the synthesis and metabo-lism of lipids including prostaglandins (PGE2) and retinoids. In addition, CYP2S1 expression is associated with patient survival in breast cancer.