CYP2D6/7 Polyklonaler Antikörper

CYP2D6/7 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17868-1-AP

Synonyme

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2D6/7, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

---

Galerie der Validierungsdaten

• 

  WB analysis using 17868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6/7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

17868-1-AP bindet in WB, IHC, ELISA CYP2D6/7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP2D6/7 fusion protein Ag12351
• Vollständiger Name: cytochrome P450, family 2, subfamily D, polypeptide 6
• Berechnetes Molekulargewicht: 497 aa, 56 kDa
• Beobachtetes Molekulargewicht: 48-56 kDa
• GenBank-Zugangsnummer: BC075023
• Gene symbol: CYP2D6
• Gene ID (NCBI): 1565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa. This antibody can recognize human CYP2D6/7, mouse CYP2D3/4/9/10/22/26, rat CYP2D1/3/4/10/26, due to the high homology.

Publikationen

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
human	WB	Drug Des Devel Ther; Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.; Authors - Hongfang Li
human	WB	Drug Metab Pharmacokinet; Cloning, sequence analysis, and tissue expression of marmoset paraoxonase 1.; Authors - Shotaro Uehara
human,mouse	WB	Xenobiotica; Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.; Authors - Shotaro Uehara
rat	WB	Pharmazie; Silybin enhances the blood concentration of brexpiprazole in rats by downregulating expression of CYP3A4 and CYP2D6.; Authors - Congcong Yu
		J Adv Res; Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug; Authors - Jing Guo