CYP2A6 Polyklonaler Antikörper

CYP2A6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21721-1-AP

Synonyme

Coumarin 7 hydroxylase, CYP2A, CYP2A3, CYP2A6, CYPIIA6, Cytochrome P450 2A6, Cytochrome P450 IIA3, Cytochrome P450(I), P450C2A, P450PB

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Galerie der Validierungsdaten

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  WB analysis using 21721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21721-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21721-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 21721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Mauslungengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

21721-1-AP bindet in WB, IHC, ELISA CYP2A6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP2A6 fusion protein Ag16377
• Vollständiger Name: cytochrome P450, family 2, subfamily A, polypeptide 6
• Berechnetes Molekulargewicht: 494 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC096253
• Gene symbol: CYP2A6
• Gene ID (NCBI): 1548
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biochem Pharmacol; Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells.; Authors - Ashley A Untereiner
human,mouse	WB	Xenobiotica; Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.; Authors - Shotaro Uehara
human	WB	Sci Rep; Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells; Authors - Sunitha Kodidela
mouse	WB	Front Toxicol; E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis; Authors - Bayan Alakhtar