CYP26B1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP26B1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 21555-1-AP

Synonyme

CYP26A2, CYP26B1, Cytochrome P450 26A2, Cytochrome P450 26B1, Cytochrome P450RAI 2, P450RAI 2, P450RAI2

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Galerie der Validierungsdaten

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  WB analysis of human brain using 21555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 21555-1-AP

  IP Result of anti-CYP26B1 (IP:21555-1-AP, 4ug; Detection:21555-1-AP 1:500) with L02 cells lysate 2400ug.

• 

  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP26B1 antibody (21555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

21555-1-AP bindet in WB, IP, IHC, IF, ELISA CYP26B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP26B1 fusion protein Ag15368
• Vollständiger Name: cytochrome P450, family 26, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 512 aa, 58 kDa
• Beobachtetes Molekulargewicht: 49-58 kDa
• GenBank-Zugangsnummer: BC069443
• Gene symbol: CYP26B1
• Gene ID (NCBI): 56603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).

Publikationen

Species	Application	Title
human	WB	Nat Genet; Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.; Authors - Jiang Chang ; KD Validated
mouse	IHC	Brain Struct Funct; Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1.; Authors - Hande Login
mouse	IF	J Neurosci; The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map.; Authors - Hande Login
mouse	IF	J Neurosci; Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.; Authors - Sofia Håglin
		Front Mol Neurosci; Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.; Authors - Yafang Zhang
human	WB	FASEB J; Dysregulated retinoic acid signaling in airway smooth muscle cells in asthma.; Authors - Amy E Defnet

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (02-06-2020)

The antibody stains a subset of proximal tubulues.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse kidney

CYP26B1 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:21555-1-AP)