CYP24A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP24A1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 21582-1-AP

Synonyme

24 OHase, CP24, CYP24, CYP24A1, Cytochrome P450 24A1, Cytochrome P450 CC24, P450 CC24, Vitamin D(3) 24 hydroxylase

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Galerie der Validierungsdaten

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  WB analysis of A549 using 21582-1-AP

  WB result of CYP24A1 antibody (21582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP24A1 transfected A549 cells.

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  WB analysis using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21582-1-AP

  IP Result of anti-CYP24A1 (IP:21582-1-AP, 4ug; Detection:21582-1-AP 1:300) with HeLa cells lysate 2800ug.

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  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Produktinformation

21582-1-AP bindet in WB, IP, IHC, ELISA CYP24A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP24A1 fusion protein Ag16134
• Vollständiger Name: cytochrome P450, family 24, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 514 aa, 59 kDa
• Beobachtetes Molekulargewicht: 54-59 kDa
• GenBank-Zugangsnummer: BC109083
• Gene symbol: CYP24A1
• Gene ID (NCBI): 1591
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP24A1(1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and 1-alpha,25-dihydroxyvitamin D3. Cholecalciferol is metabolised by the 25-hydroxylases (CYP2R1 and CYP27A1) in the liver, before 25-hydroxycholecalciferol (25(OH)D3) relocates to the circulation (PMID:20362668). The gene CYP24A1 has three isoforms, for the transit peptide "mitochondrial domain" removed, the MW of CYP24A1 will be 55-59 kDa, 47-51 kDa and 43 kDa. This antibody is specific to CYP24A1.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Lett; TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.; Authors - Heng Xiao ; KD Validated
mouse	WB	Acta Physiol (Oxf); Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.; Authors - Eva Maria Pastor-Arroyo
mouse	WB	J Bone Miner Res; Exercise May Ameliorate the Detrimental Side Effects of High Vitamin D Supplementation on Muscle Function in Mice.; Authors - Danielle A Debruin
human	WB	Cancer Commun (Lond); Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.; Authors - Luwei He
human	WB, IHC	J Steroid Biochem Mol Biol; Eldecalcitol (ED-71), an Analog of 1α, 25-dihydroxy Vitamin D3 as a Potential Anti-cancer Agent for Oral Squamous Cell Carcinomas.; Authors - T Shintani
mouse	WB	J Physiol; 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine.; Authors - Nati Hernando

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-30-2023)

This antibody appeared to work pretty well for immunofluorescence in mouse tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Kidney