CYP11A1 Monoklonaler Antikörper

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• KD/KO Validated

CYP11A1 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1C5D5

Kat-Nr. : 67264-1-Ig

Synonyme

Cholesterol desmolase, CYP11A, CYP11A1, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), P450SCC

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 67264-1-Ig

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells.

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  WB analysis of human placenta using 67264-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67264-1-Ig

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 67264-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67264-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Caco-2-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

67264-1-Ig bindet in WB, IHC, ELISA CYP11A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP11A1 fusion protein Ag4353
• Vollständiger Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 521 aa, 60 kDa
• Beobachtetes Molekulargewicht: 49-52 kDa
• GenBank-Zugangsnummer: BC032329
• Gene symbol: CYP11A1
• Gene ID (NCBI): 1583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,rat	WB	Front Neurosci; Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats.; Authors - Tianying Pan
human	IHC	Reprod Sci; Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis; Authors - Haibo Zhang
		Cell Rep; Dissecting the spermatogonial stem cell niche using spatial transcriptomics; Authors - Shreya Rajachandran
mouse	IHC,WB	Toxicol Appl Pharmacol; Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice; Authors - Yong Zou
human	WB	Mol Hum Reprod; Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction; Authors - Maria Höfner