CYFIP1/2 Polyklonaler Antikörper

CYFIP1/2 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 16011-1-AP

Synonyme

CYFIP1/2, CYFIP2, KIAA1168, p53 inducible protein 121, PIR121

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Galerie der Validierungsdaten

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  WB analysis of human brain using 16011-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16011-1-AP

  IP Result of anti-CYFIP2 (IP:16011-1-AP, 4ug; Detection:16011-1-AP 1:700) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 16011-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CYFIP1/2 (16011-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16011-1-AP bindet in WB, IP, IHC, FC, ELISA CYFIP1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYFIP1/2 fusion protein Ag8749
• Vollständiger Name: cytoplasmic FMR1 interacting protein 2
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC011762
• Gene symbol: CYFIP2
• Gene ID (NCBI): 26999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

Publikationen

Species	Application	Title
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB. Dilution 1:1000 in TBS-T 5% BSA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuronal cells