CYB5R3 Monoklonaler Antikörper

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• KD/KO Validated

CYB5R3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

9C4D10

Kat-Nr. : 66022-1-Ig

Synonyme

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66022-1-Ig

  WB results of CYB5R3 antibody (66022-1-Ig, 1:60000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis of HeLa using 66022-1-Ig

  Western blot analysis of CYB5R3-specific in various tissues using Proteintech antibody 66022-1-Ig at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66022-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66022-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66022-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66022-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66022-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66022-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66022-1-Ig (CYB5R3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66022-1-Ig (CYB5R3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66022-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66022-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 66022-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYB5R3 antibody (66022-1-Ig, Clone: 9C4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66022-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66022-1-Ig(CYB5R3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Plasmagewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66022-1-Ig bindet in WB, IHC, IF, ELISA CYB5R3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYB5R3 fusion protein Ag18096
• Vollständiger Name: cytochrome b5 reductase 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC004821
• Gene symbol: CYB5R3
• Gene ID (NCBI): 1727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.