CXXC5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXXC5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 16513-1-AP

Synonyme

CXXC finger 5, CXXC5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF, TCCCIA00297

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 16513-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16513-1-PBS

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 16513-1-AP

  IP result of anti-CXXC5(IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:8000) with SH-SY5Y cells lysate 1240 ug.

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  IP experiment of SH-SY5Y using 16513-1-AP

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug.

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  IHC staining of human prostate cancer using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16513-1-AP (CXXC5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, MCF-7-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

16513-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA CXXC5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXXC5 fusion protein Ag9733
• Vollständiger Name: CXXC finger 5
• Berechnetes Molekulargewicht: 322aa,33 kDa; 227aa,24 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC017439
• Gene symbol: CXXC5
• Gene ID (NCBI): 51523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

Publikationen

Species	Application	Title
human	ChIP,IHC,WB	Nat Commun; A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.; Authors - Yundong He ; KD Validated
mouse	WB	J Exp Med; Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.; Authors - Shixin Ma ; KO Validated
human	WB	Oncogene; NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.; Authors - Sheng Tan ; KD Validated
human	WB,ChIP	Haematologica; The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion.; Authors - Audrey Astori
human	WB	Sci Rep; KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors.; Authors - Zhibin Cui
human,mouse	WB,ChIP	Front Cell Dev Biol; Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation.; Authors - Xiaoyan Wu