CXCR4 Monoklonaler Antikörper

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• KD/KO Validated

CXCR4 Monoklonal Antikörper für IHC

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus

Anwendung

IHC

Konjugation

Biotin

CloneNo.

4B5E4

Kat-Nr. : Biotin-60042

Synonyme

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-60042 bindet in IHC CXCR4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CXCR4 fusion protein Ag1528
• Vollständiger Name: chemokine (C-X-C motif) receptor 4
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 60~70 kDa
• GenBank-Zugangsnummer: BC020968
• Gene symbol: CXCR4
• Gene ID (NCBI): 7852
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).