Cathepsin D Polyklonaler Antikörper

Cathepsin D Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 55021-1-AP

Synonyme

Cathepsin D, CLN10, CPSD, CTSD

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Galerie der Validierungsdaten

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  WB analysis using 55021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55021-1-AP (Cathepsin D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

55021-1-AP bindet in WB, IHC, IF, ELISA Cathepsin D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_001909
• Gene symbol: CTSD
• Gene ID (NCBI): 1509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Publikationen

Species	Application	Title
mouse	IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Toxicology; 1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.; Authors - Jing Lu
human	IF	Pathogens; Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species; Authors - Kayla M Fantone
human	WB	Cell Death Discov; A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.; Authors - Pengyun Li
human	WB	Chem Biol Interact; In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.; Authors - Jin Lei
human	WB	Biochem Biophys Res Commun; Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation; Authors - Weigang Yuan