Alpha E-Catenin Monoklonaler Antikörper

Alpha E-Catenin Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2B7B8

Kat-Nr. : 66221-1-Ig

Synonyme

Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 66221-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66221-1-Ig (Alpha E catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

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  FC experiment of MCF-7 using 66221-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Alpha E-Catenin (66221-1-Ig, Clone:2B7B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

66221-1-Ig bindet in WB, IHC, FC, ELISA Alpha E-Catenin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha E-Catenin fusion protein Ag23603
• Vollständiger Name: catenin (cadherin-associated protein), alpha 1, 102kDa
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC031262
• Gene symbol: CTNNA1
• Gene ID (NCBI): 1495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publikationen

Species	Application	Title
mouse	IHC	Int J Mol Sci; Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.; Authors - Chisato Sakuma
human	WB	Oncotarget; YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.; Authors - Dan Sun
human	WB,IHC	Mol Med Rep; Wild-type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β-catenin pathway; Authors - Xianhua Hu