CTCF Polyklonaler Antikörper

CTCF Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30428-1-AP

Synonyme

11 zinc finger protein, CCCTC binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF

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Galerie der Validierungsdaten

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  WB analysis using 30428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30428-1-AP (CTCF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 30428-1-AP

  IP result of anti-CTCF(IP:30428-1-AP, 4ug; Detection:30428-1-AP 1:8000) with MCF-7 cells lysate 1880 ug.

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  IHC staining of mouse lung using 30428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30428-1-AP (CTCF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 30428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30428-1-AP (CTCF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30428-1-AP bindet in WB, IP, IHC, ELISA CTCF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTCF fusion protein Ag32417
• Vollständiger Name: CCCTC-binding factor (zinc finger protein)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 110-150 kDa
• GenBank-Zugangsnummer: BC014267
• Gene symbol: CTCF
• Gene ID (NCBI): 10664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcriptional insulators are DNA elements that set boundaries on the actions of enhancer and silencer elements and thereby organize the eukaryotic genome into regulatory domains. All vertebrate insulators appear to use the versatile CTCF protein. CTCF uses various combinations of its 11 zinc fingers to recognize a variety of unrelated DNA sequences. Once bound to DNA, CTCF can function as a transcriptional insulator, repressor, or activator, depending on the context of the binding site [PMID:12787766,15454938]. In vertebrates, this 11 zinc-finger protein is shown to be crucial in processes of epigenetic imprinting, X chromosome inactivation , and associated with various complex human diseases including cancer and diabetes [PMID:23139640]. The calcualted molecular weight of CTCF is 83 kDa, but stimulation of human corneal epithelial cells with hypoxic stress suppressed a high molecular mass form of CTCF (150 kDa), but not a lower molecular weight form of CTCF (130 kDa)(PMID: 22354964), and there are multiple isoforms of CTCF with molecular masses of 55, 70, 73, 80, 97, and 130 kDa have been observed (PMID: 12878173).