FLJ23584 Polyklonaler Antikörper

FLJ23584 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21143-1-AP

Synonyme

C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 21143-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21143-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21143-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21143-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21143-1-AP

  Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hirngewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21143-1-AP bindet in WB, IHC, IF,ELISA FLJ23584 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FLJ23584 fusion protein Ag15647
• Vollständiger Name: hypothetical FLJ23584
• Berechnetes Molekulargewicht: 234 aa, 26 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC007210
• Gene symbol: CTA-216E10.6
• Gene ID (NCBI): 79640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.