CSK Monoklonaler Antikörper

CSK Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3B12C5

Kat-Nr. : 66115-1-Ig

Synonyme

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66115-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66115-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66115-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66115-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 66115-1-Ig

  Immunofluorescent analysis of A431 cells using 66115-1-Ig (CSK antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66115-1-Ig bindet in WB, IF, ELISA CSK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSK fusion protein Ag12103
• Vollständiger Name: c-src tyrosine kinase
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC106073
• Gene symbol: CSK
• Gene ID (NCBI): 1445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

Publikationen

Species	Application	Title
mouse	WB	Andrology; Trps1 targets Ccnd1 to regulate mouse Leydig cell proliferation.; Authors - Jiandong Sun