GM-CSF Polyklonaler Antikörper

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• KD/KO Validated

GM-CSF Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 17762-1-AP

Synonyme

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

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Galerie der Validierungsdaten

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  WB analysis using 17762-1-AP

  PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 17762-1-AP (GM-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

17762-1-AP bindet in WB, IHC, IF, ELISA GM-CSF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GM-CSF fusion protein Ag12142
• Vollständiger Name: colony stimulating factor 2 (granulocyte-macrophage)
• Berechnetes Molekulargewicht: 144 aa, 16 kDa
• GenBank-Zugangsnummer: BC108724
• Gene symbol: CSF2
• Gene ID (NCBI): 1437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Publikationen

Species	Application	Title
human	WB,IHC	J Exp Med; Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma.; Authors - Jing Liu
mouse	IF	J Am Soc Nephrol; Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury.; Authors - Leyuan Xu
human,mouse	WB	Oncogene; CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer.; Authors - Hsiang-Wei Huang
mouse	WB	Int J Mol Sci; Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.; Authors - Tao Wang
human	WB,IHC	FEBS J; Myeloid-derived suppressor cells promote epithelial ovarian cancer cell stemness by inducing the CSF2/p-STAT3 signalling pathway.; Authors - Xiaofeng Li ; KD Validated
human		J Virol; RNA helicase A is an important host factor involved in dengue virus replication.; Authors - Yi Wang