CRYBB2 Polyklonaler Antikörper

CRYBB2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18154-1-AP

Synonyme

Beta B2 crystallin, Beta crystallin B2, Beta crystallin Bp, CCA2, CRYB2, CRYB2A, CRYBB2, crystallin, beta B2, D22S665

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Galerie der Validierungsdaten

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  WB analysis using 18154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18154-1-AP (CRYBB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18154-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18154-1-AP (CRYBB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 18154-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 18154-1-AP (CRYBB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18154-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18154-1-AP (CRYBB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, fetales humanes Hirngewebe, Maushirngewebe, Ratten-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

18154-1-AP bindet in WB, ELISA CRYBB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRYBB2 fusion protein Ag12906
• Vollständiger Name: crystallin, beta B2
• Berechnetes Molekulargewicht: 205 aa, 23 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC069535
• Gene symbol: CRYBB2
• Gene ID (NCBI): 1415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cells; Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2).; Authors - Naoki Yamamoto
		Invest Ophthalmol Vis Sci; Relationship Between the Altered Expression and Epigenetics of GSTM3 and Age-Related Cataract.; Authors - Bo Li
zebrafish	WB	FASEB J; Knockout of miR-184 in zebrafish leads to ocular abnormalities by elevating p21 levels; Authors - Jing Zhang