CRMP1 Monoklonaler Antikörper

CRMP1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4G5

Kat-Nr. : 68021-1-Ig

Synonyme

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse cerebellum using 68021-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68021-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 68021-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 68021-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68021-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of chicken brain using 68021-1-Ig

  chicken brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 68021-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit cerebellum using 68021-1-Ig

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 68021-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68021-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Hühnerhirngewebe, Kaninchen-Cerebellum-Gewebe, Kaninchenhirngewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68021-1-Ig bindet in WB, IHC, ELISA CRMP1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRMP1 fusion protein Ag30090
• Vollständiger Name: collapsin response mediator protein 1
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC000252
• Gene symbol: CRMP1
• Gene ID (NCBI): 1400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.