CRM1 Polyklonaler Antikörper

CRM1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 27917-1-AP

Synonyme

CRM1, Exp1, Exportin 1, XPO1

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Galerie der Validierungsdaten

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  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 27917-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, HT-1080.Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27917-1-AP bindet in WB, IHC, IF, ELISA CRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRM1 fusion protein Ag27207
• Vollständiger Name: exportin 1 (CRM1 homolog, yeast)
• Berechnetes Molekulargewicht: 1071 aa, 123 kDa
• Beobachtetes Molekulargewicht: 123 kDa
• GenBank-Zugangsnummer: BC032847
• Gene symbol: XPO1
• Gene ID (NCBI): 7514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publikationen

Species	Application	Title
human	IF	J Cell Commun Signal; CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles; Authors - Sukhbir Kaur
mouse	IHC	J Cancer Res Clin Oncol; Selinexor demonstrates anti-tumor efficacy in paired patient-derived xenograft models and hydrogel-embedded histoculture drug sensitivity test of penile cancer; Authors - Yuanqiao He
human	WB	Mol Cell; EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma; Authors - Deguan Lv
mouse	WB	Mol Cell; A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity; Authors - Xu Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was pretty good

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

CRM1 Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:27917-1-AP)

FH

Wei (Verified Customer) (03-12-2020)

strong, clear band for mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart