CRLS1 Polyklonaler Antikörper

CRLS1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 51055-1-AP

Synonyme

C20orf155, Cardiolipin synthase, cardiolipin synthase 1, CLS, CLS1, CRLS1, GCD10, Protein GCD10 homolog

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 51055-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51055-1-AP (CRLS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51055-1-AP (CRLS1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Mammakarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

51055-1-AP bindet in WB, IHC, ELISA CRLS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cardiolipin synthase 1
• Berechnetes Molekulargewicht: 301 aa, 32 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC069010
• Gene symbol: CRLS1
• Gene ID (NCBI): 54675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cardiolipin (CL) is a mitochondrial specific double negatively charged phospholipid which is intricately involved in regulating bioenergetic efficiency. In eukaryotes, CL is synthesized by cardiolipin synthase (CRLS1 or CLS1) which catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form CL and glycerol. Recently it has been reported that transgenic expression of CRLS1 accelerates cardiolipin remodeling, improves mitochondrial function, modulates mitochondrial signaling, and attenuates mitochondrial dysfunction during diabetes, thereby identifying CRLS1 as a novel therapeutic target to attenuate mitochondrial dysfunction in diabetic myocardium. The predicted molecular weight of CRLS1 is around 32-35 kDa, while a 50 kDa protein with CRLS1 activity had been observed in liver isolation, which may represent an isoform of CRLS1 (20652826).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice; Authors - Hongming Su
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen ; KD Validated