CRK Polyklonaler Antikörper

CRK Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16685-1-AP

Synonyme

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

---

Galerie der Validierungsdaten

• 

  WB analysis of NIH/3T3 using 16685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16685-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16685-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 16685-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 16685-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16685-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 16685-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A549-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16685-1-AP bindet in WB, IHC, IF,ELISA CRK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRK fusion protein Ag10016
• Vollständiger Name: v-crk sarcoma virus CT10 oncogene homolog (avian)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008506
• Gene symbol: CRK
• Gene ID (NCBI): 1398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.