CRISP3 Polyklonaler Antikörper

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• KD/KO Validated

CRISP3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14847-1-AP

Synonyme

Aeg2, CRISP 3, CRISP3, CRS3, SGP28

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Galerie der Validierungsdaten

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  WB analysis using 14847-1-AP

  human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14847-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14847-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 14847-1-AP

  IP Result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug.

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, humanes Plazenta-Gewebe, Jurkat-Zellen, Maus-Eierstockgewebe, Maus-Kolongewebe, Maus-Thymusgewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

14847-1-AP bindet in WB, IP, IHC, ELISA CRISP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cysteine-rich secretory protein 3
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_006061
• Gene symbol: CRISP3
• Gene ID (NCBI): 10321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Publikationen

Species	Application	Title
human	IHC	J Natl Cancer Inst; Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness.; Authors - Paola A Rojas
human	IHC	J Hematol Oncol; Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis.; Authors - Samir Al Bashir
human	WB,IHC	Int J Biol Sci; Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis; Authors - Yu-Ming Wang ; KD Validated
human	WB	J Dent Res; Proteome Analysis of Temporomandibular Joint with Disc Displacement; Authors - X Liu