CREB3L2 Polyklonaler Antikörper

CREB3L2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14514-1-AP

Synonyme

BBF2H7, CREB3L2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 14514-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14514-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14514-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14514-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14514-1-AP (CREB3L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushodengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

14514-1-AP bindet in WB, IF, ELISA CREB3L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREB3L2 fusion protein Ag5973
• Vollständiger Name: cAMP responsive element binding protein 3-like 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC063666
• Gene symbol: CREB3L2
• Gene ID (NCBI): 64764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.

Publikationen

Species	Application	Title
human	WB	Br J Pharmacol; Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78).; Authors - Yongli Zhang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IF	J Invest Dermatol; BBF2H7-mediated Sec23A pathway is required for endoplasmic reticulum-to-Golgi trafficking in dermal fibroblasts to promote collagen synthesis.; Authors - Ishikura-Kinoshita Seiko S
human	WB	J Biol Chem; APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.; Authors - Liu Chao C
human	WB	Mol Biol Rep; Expression analysis and functional characterization of thioredoxin domain-containing protein 11; Authors - Ryoichi Murase