CREB3 Monoklonaler Antikörper

CREB3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12H11

Kat-Nr. : 68379-1-Ig

Synonyme

CREB 3, CREB3, LUMAN, LZIP

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Galerie der Validierungsdaten

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  WB analysis of T-47D using 68379-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 68379-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 68379-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HCT 116-Zellen, hTERT-RPE1-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68379-1-Ig bindet in WB, IF, ELISA CREB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CREB3 fusion protein Ag1823
• Vollständiger Name: cAMP responsive element binding protein 3
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 40-64 kDa
• GenBank-Zugangsnummer: BC009402
• Gene symbol: CREB3
• Gene ID (NCBI): 10488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).