CREB1 Monoklonaler Antikörper
CREB1 Monoklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1E11C1
Kat-Nr. : 67927-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen |
Erfolgreiche Detektion in IHC | humanes Prostatakarzinomgewebe, humanes Zervixkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | A431-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF) | IF : 1:4000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 2 publications below |
Produktinformation
67927-1-Ig bindet in WB, IHC, IF, ELISA CREB1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | CREB1 fusion protein Ag2852 |
Vollständiger Name | cAMP responsive element binding protein 1 |
Berechnetes Molekulargewicht | 341 aa, 35 kDa |
Beobachtetes Molekulargewicht | 43-46 kDa |
GenBank-Zugangsnummer | BC010636 |
Gene symbol | CREB1 |
Gene ID (NCBI) | 1385 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
Protokolle
Produktspezifische Protokolle | |
---|---|
WB protocol for CREB1 antibody 67927-1-Ig | Protokoll herunterladen |
IHC protocol for CREB1 antibody 67927-1-Ig | Protokoll herunterladen |
IF protocol for CREB1 antibody 67927-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
ACS Chem Neurosci Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus | ||
BMC Cancer Identification of TLN1 as a prognostic biomarker to effect cell proliferation and differentiation in acute myeloid leukemia |