CRAT Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CRAT Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 15170-1-AP

Synonyme

CACP, Carnitine acetylase, carnitine acetyltransferase, Carnitine O acetyltransferase, CAT, CAT1, CRAT

---

Galerie der Validierungsdaten

• 

  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 15170-1-AP

  IP Result of anti-CRAT (IP:15170-1-AP, 4ug; Detection:15170-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below

Produktinformation

15170-1-AP bindet in WB, IP, ELISA CRAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRAT fusion protein Ag7422
• Vollständiger Name: carnitine acetyltransferase
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 55-68 kDa
• GenBank-Zugangsnummer: BC000723
• Gene symbol: CRAT
• Gene ID (NCBI): 1384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRAT, also named as CAT1, belongs to the carnitine/choline acetyltransferase family. It is specific for short chain fatty acids. CRAT seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CRAT is an active forms for carnitine acetyltransferase. This antibody can bind the close sequences genes.

Publikationen

Species	Application	Title
rat	WB	Cell Metab; Dissociation of Muscle Insulin Resistance from Alterations in Mitochondrial Substrate Preference.; Authors - Joongyu D Song
		Cancer Res; Organelle-Derived Acetyl-CoA Promotes Prostate Cancer Cell Survival, Migration, and Metastasis via Activation of Calmodulin Kinase II.; Authors - Guoyu Yu
goat	WB	Redox Biol; TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis.; Authors - Xutong Sun ; KD Validated
sheep	WB	Antioxid Redox Signal; Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow.; Authors - Sun Xutong X
mouse	WB	Mol Metab; Carnitine acetyltransferase (CRAT) expression in macrophages is dispensable for nutrient stress sensing and inflammation.; Authors - Emily L Goldberg
mouse	WB	Heliyon; Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction; Authors - Yasuro Furuichi