CD35 Monoklonaler Antikörper

CD35 Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B8E6

Kat-Nr. : 68033-1-Ig

Synonyme

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using 68033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 68033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using 68033-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD35 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human appendicitis using 68033-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CR1 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Appendizitis-Gewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:550-1:2200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68033-1-Ig bindet in IHC, IF, ELISA CD35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD35 fusion protein Ag30268
• Vollständiger Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Berechnetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: NM_000573
• Gene symbol: CR1
• Gene ID (NCBI): 1378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).