CPSF4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CPSF4 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 15023-1-AP

Synonyme

CPSF 30 kDa subunit, CPSF30, CPSF4, NAR, Neb 1, NEB1, No arches homolog

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 15023-1-AP

  WB result of CPSF4 antibody (15023-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPSF4 transfected HEK-293 cells.

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  WB analysis of Jurkat using 15023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15023-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15023-1-AP

  IP Result of anti-CPSF4 (IP:15023-1-AP, 3ug; Detection:15023-1-AP 1:400) with HeLa cells lysate 1000ug.

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15023-1-AP

  Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody 15023-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

15023-1-AP bindet in WB, IP, IHC, IF, ELISA CPSF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF4 fusion protein Ag7103
• Vollständiger Name: cleavage and polyadenylation specific factor 4, 30kDa
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC003101
• Gene symbol: CPSF4
• Gene ID (NCBI): 10898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.

Publikationen

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB,IP	Mol Oncol; CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.; Authors - Wangbing Chen ; KD Validated
human	WB,IHC,IF	Cancer Lett; Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.; Authors - Canhui Yi ; KD Validated
human	WB,IF	Mol Oncol; CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway.; Authors - Zhipeng Tang
pig	WB	Int J Biol Macromol; Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.; Authors - Zhaohui Teng
human	WB, IF	Biochim Biophys Acta Mol Cell Res; Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.; Authors - Qian Yang