CPS1 Polyklonaler Antikörper

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CPS1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24513-1-AP

Synonyme

Carbamoyl-phosphate synthase [ammonia], mitochondrial, Carbamoyl-phosphate synthetase I, CPSase I, EC:6.3.4.16

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  WB analysis of HeLa using 24513-1-AP

  WB result of CPS1 antibody (24513-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPS1 transfected HeLa cells.

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  WB analysis of HepG2 using 24513-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 24513-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24513-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24513-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24513-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24513-1-AP bindet in WB, IHC, ELISA CPS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPS1 fusion protein Ag21605
• Vollständiger Name: carbamoyl-phosphate synthetase 1, mitochondrial
• Berechnetes Molekulargewicht: 1500 aa, 165 kDa
• Beobachtetes Molekulargewicht: 140-165 kDa
• GenBank-Zugangsnummer: BC140943
• Gene symbol: CPS1
• Gene ID (NCBI): 1373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.