CPA4 Polyklonaler Antikörper

CPA4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 26824-1-AP

Synonyme

Carboxypeptidase A3, carboxypeptidase A4, CPA3, CPA4

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 26824-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26824-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26824-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 26824-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26824-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, A549-Zellen, HepG2-Zellen, Mausnierengewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

26824-1-AP bindet in WB, IHC, ELISA CPA4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPA4 fusion protein Ag25327
• Vollständiger Name: carboxypeptidase A4
• Berechnetes Molekulargewicht: 421 aa, 47 kDa
• Beobachtetes Molekulargewicht: 44 kDa, 150 kDa
• GenBank-Zugangsnummer: BC052289
• Gene symbol: CPA4
• Gene ID (NCBI): 51200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carboxypeptidase A4 (CPA4), a member of the metallocarboxypeptidase family, is a zinc-containing exopeptidase that catalyzes the release of carboxy-terminal amino acids. CPA4 was previously shown to play a crucial role in cell growth and differentiation by modulating or inactivating peptide hormone activity (PMID: 27904708). CPA4 expression is increased in multiple cancer tissues, including tissues from patients with pancreatic cancer, gastric cancer, esophageal squamous cell carcinoma, and lung cancer, and may serve as a potential diagnostic and prognostic marker (PMID: 31397502, 32922037).

Publikationen

Species	Application	Title
human	WB	Sci Signal; Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling.; Authors - Fernanda G Kugeratski
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); Angiopoietin-1 promotes triple-negative breast cancer cell proliferation by upregulating carboxypeptidase A4; Authors - Xue Liu
human,mouse	WB	Cell Death Dis; Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathway; Authors - Luoling Wang