COQ5 Polyklonaler Antikörper

COQ5 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17453-1-AP

Synonyme

COQ5

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17453-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17453-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

17453-1-AP bindet in WB, IHC, IF, ELISA COQ5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COQ5 fusion protein Ag10203
• Vollständiger Name: coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
• Berechnetes Molekulargewicht: 327 aa, 37 kDa
• Beobachtetes Molekulargewicht: 34.7 kDa
• GenBank-Zugangsnummer: BC107874
• Gene symbol: COQ5
• Gene ID (NCBI): 84274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	J Exp Med; Implication of folate deficiency in CYP2U1 loss of function.; Authors - Claire Pujol
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
human	WB	J Am Soc Nephrol; ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.; Authors - Eugen Widmeier
mouse	WB	EMBO Mol Med; The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.; Authors - Marta Luna-Sánchez
mouse	WB	Sci Rep; Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.; Authors - María Rodríguez-Hidalgo