COQ4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COQ4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16654-1-AP

Synonyme

CGI 92, COQ4

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16654-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16654-1-AP (COQ4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 16654-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16654-1-AP (COQ4 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16654-1-AP

  IP result of anti-COQ4 (IP:16654-1-AP, 4ug; Detection:16654-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 16654-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16654-1-AP (COQ4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

16654-1-AP bindet in WB, IP, IHC, ELISA COQ4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COQ4 fusion protein Ag10089
• Vollständiger Name: coenzyme Q4 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 265 aa, 30 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC011895
• Gene symbol: COQ4
• Gene ID (NCBI): 51117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human coenzyme Q4 (COQ4), as a component of the coenzyme Q biosynthetic pathway, is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells (PMID: 38295803). COQ4 deficiency manifests as an early-onset neurodegenerative disorder. COQ4 encodes a ubiquitously expressed 265-amino-acid protein that is peripherally associated with the mitochondrial inner membrane on the matrix side (PMID: 25658047, 34656997)

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
human	WB	Ann Clin Transl Neurol; 4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency.; Authors - Diran Herebian
human	WB	J Med Genet; Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.; Authors - Lucia Laugwitz
human	WB	Biochim Biophys Acta Bioenerg; Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability.; Authors - Hsiu-Chuan Yen
human	WB	Mol Genet Metab; Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.; Authors - Diran Herebian ; KD Validated