COMMD5 Monoklonaler Antikörper

COMMD5 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1E10G6

Kat-Nr. : 67043-1-Ig

Synonyme

COMM domain containing 5, COMMD5, FLJ13008, HCARG, HT002

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Galerie der Validierungsdaten

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  WB analysis of human heart using 67043-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67043-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67043-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67043-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, HEK-293-Zellen, MCF-7-Zellen, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67043-1-Ig bindet in WB, ELISA COMMD5 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COMMD5 fusion protein Ag18464
• Vollständiger Name: COMM domain containing 5
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC003055
• Gene symbol: COMMD5
• Gene ID (NCBI): 28991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Publikationen

Species	Application	Title
human	WB	Cell; Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome; Authors - Michael D Healy
human	WB	Aging (Albany NY); TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs); Authors - Jianyun Liu