CNRIP1 Polyklonaler Antikörper

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• KD/KO Validated

CNRIP1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16827-1-AP

Synonyme

C2orf32, CNRIP1, CRIP 1, CRIP1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 16827-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16827-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

16827-1-AP bindet in WB, IHC, ELISA CNRIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNRIP1 fusion protein Ag10428
• Vollständiger Name: cannabinoid receptor interacting protein 1
• Berechnetes Molekulargewicht: 164 aa, 19 kDa
• Beobachtetes Molekulargewicht: 13-25 kDa
• GenBank-Zugangsnummer: BC011535
• Gene symbol: CNRIP1
• Gene ID (NCBI): 25927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNRIP1, also named as C2orf32, has two isoforms with MW 13-25 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Hepatology; DNA Methylation of Cannabinoid Receptor Interacting Protein 1 Promotes Pathogenesis of Intrahepatic Cholangiocarcinoma Through Suppressing Parkin-Dependent Pyruvate Kinase M2 Ubiquitination.; Authors - Diyu Chen
mouse	WB,IHC	Brain Res Bull; Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice.; Authors - Fu Yang ; KO Validated